Metagenomics Analysis to Investigate the Microbial Communities and Their Functional Profile During Cyanobacterial Blooms in Lake Varese. in Microbial ecology / Microb Ecol. 2022 May;83(4):850-868. doi: 10.1007/s00248-021-01914-5. Epub 2021 Nov 12.

2022
ARPA Piemonte

Tipo pubblicazione

Journal Article

Autori/Collaboratori (11)Vedi tutti...

Genoni P
ARPA, Agenzia Regionale Per La Protezione Dell'Ambiente Della Lombardia, Via Ippolito Rosellini 17, 20124, Milano, Italy.
Pandolfi F
ARPA, Agenzia Regionale Per La Protezione Dell'Ambiente Della Lombardia, Via Ippolito Rosellini 17, 20124, Milano, Italy.
Skejo H
European Commission, Joint Research Centre (JRC), Via E. Fermi 2749, 21027, Ispra, VA, Italy.

et alii...

Abstract

Toxic cyanobacterial blooms represent a natural phenomenon caused by a mass proliferation of photosynthetic prokaryotic microorganisms in water environments. Bloom events have been increasingly reported worldwide and their occurrence can pose serious threats to aquatic organisms and human health. In this study, we assessed the microbial composition, with a focus on Cyanobacteria, in Lake Varese, a eutrophic lake located in northern Italy. Water samples were collected and used for obtaining a 16S-based taxonomic profile and performing a shotgun sequencing analysis. The phyla found to exhibit the greatest relative abundance in the lake included Proteobacteria, Cyanobacteria, Actinobacteriota and Bacteroidota. In the epilimnion and at 2.5?×?Secchi depth, Cyanobacteria were found to be more abundant compared to the low levels detected at greater depths. The blooms appear to be dominated mainly by the species Lyngbya robusta, and a specific functional profile was identified, suggesting that distinct metabolic processes characterized the bacterial population along the water column. Finally, analysis of the shotgun data also indicated the presence of a large and diverse phage population.

Accesso banca dati bibliografica

PMID : 34766210

DOI : 10.1007/s00248-021-01914-5

Keywords

Water quality; Microbial populations; Metagenomics; Lyngbya; Freshwater; Algal bloom; Water/analysis; Microbiota/genetics; Metagenomics; Lakes/microbiology; Humans; Eutrophication; Cyanobacteria/genetics;